Characterization of non-solvent- and thermal-induced phase separation applied polycaprolactone/demineralized bone matrix scaffold for bone tissue engineering

Polycaprolactone (PCL) is a widely applied biomaterial in bone tissue engineering (BTE) due to its superior mechanical properties and biodegradability. However, the high hydrophobicity low cell adhesion of PCL show limited interactions. Herein, we prepared porous PCL/DBP composites with improved through addition demineralized powder (DBP). Three-dimensional scaffolds were fabricated by mixing various concentrations DBP applying non-solvent-induced phase separation (NIPS) thermal-induced (TIPS) (N-TIPS) solvent casting particulate leaching (SCPL) impart porosity. A characteristic evaluation was performed X-ray diffraction (XRD), morphological analysis, physicochemical bioactivity test, test. Upon culture mouse marrow stem cells (mBMSCs), proliferation osteogenic differentiation mBMSC evaluated using quantitative dsDNA analysis alkaline phosphatase (ALP) activity, respectively. The scaffold formed large amount hydroxyapatite (HAp). Also, increased enhancing adhesion. could provide favorable microenvironment for be promising model engineering.